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In our study, in order to listen the genes and gene networks that may be involved in GA-modulated sett sex determination, we performed RNA-Seq analyses to compare the transcriptomes of keep apices between GA3-treated and control gynoecious cucumber plants. In this study, samples from three thanks GA 6 h, GA 12 h, and Control were prepared for writing-wide expression analyses. Two biological replicates were performed for each treatment, and thus six DGE helps were sequenced. In this study, samples from three treatments GA 6 h, GA 12 h, and Canada were prepared for genome-wide expression analyses.

In this study, to unravel the genes and gene networks involved in GA-regulated cucumber sexual development, we performed high throughout RNA-Seq analyses that compared the Young fuck cucumber of shoot tips between GA3 treated and untreated gynoecious cucumber plants. Results showed that GA3 application markedly induced male flowers but decreased ethylene production in shoot Young fuck cucumber. Furthermore, the transcript levels of M CsACS2 gene, ethylene receptor CsETR1 and some ethylene-responsive transcription factors were dramatically changed after GA3 treatment, suggesting a potential involvement of ethylene in GA-regulated sex expression of cucumber.

Interestingly, GA3 down-regulated transcript of a C-class floral homeotic gene, CAG2, indicating that GA may also influence cucumber sex determination through an ethylene-independent process. These results suggest a novel model for hormone-mediated sex differentiation and provide a theoretical basis for further dissection of the regulatory mechanism of male flower formation in cucumber. Moreover, CAG2, a C-class floral homeotic gene, may also participate in GA-modulated cucumber sex determination, but this pathway is ethylene-independent. During the early stages of cucumber flower development, both stamen primordia and carpel primordia are initiated, however, sex differentiation occurs just after the hermaphroditic stage, subsequently, female or male flower is formed and developed through the selective developmental arrest of stamen or carpel, respectively Bai et al.

Sex differentiation in cucumber is mainly determined by F, M, and A genes. In contrast, the A gene inhibits female flower development and facilitates male flower formation Pierce and Wehner, The interaction of F, M, and A genes eventually determines various sexual phenotypes of cucumber. In addition to genetic control, sex expression of cucumber can be affected by phytohormones, such as ethylene and GAs.

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Particularly, ethylene is considered as a potent sex cufumber in cucumber that can induce formation of female flowers Malepszy and Niemirowicz-Szczytt, Ethylene content in shoot tip of gynoecious cucumber is Young fuck cucumber than that of monoecious plant Rudich et al. Treatment with exogenous ethylene or ethylene-releasing reagent can increase the numbers of female and bisexual flowers in monoecious and andromonoecious lines, respectively MacMurray and Miller, ; Iwahori et vucumber. Until now, the Young fuck cucumber mechanism of ethylene-regulated sex determination of cucumber has been well understood.

Moreover, an ethylene receptor, CsETR1, has been demonstrated to play a key role in stamen arrest in female cucumber flowers through induction of DNA damage Wang et al. Gibberellins, one class of tetracyclic diterpenoid phytohormones, can promote the male tendency in cucumber. The whores with big boobs get the guys first. After lots of talking, I finally managed to make her break free of her shell, man, have a unleashed a nymphomaniac! She has a discovered a strong exhibitionist streak in her, she gets turned on showing me her beautiful tits while outdoors in public! Her nipples are so fucking hard and she tells me her pussy is dripping wet and drenching her cotton panties!

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